Effect of vascular endothelial growth factor C (VEGF-C) gene transfer in rat model of secondary lymphedema.
Identifieur interne : 012D80 ( Main/Exploration ); précédent : 012D79; suivant : 012D81Effect of vascular endothelial growth factor C (VEGF-C) gene transfer in rat model of secondary lymphedema.
Auteurs : Yanli Liu [République populaire de Chine] ; Yunhai Fang ; Ping Dong ; Jie Gao ; Rong Liu ; Hhahbaz M ; Yushun Bi ; Zhaoxi Ding ; Hua Tian ; Zhiyu LiuSource :
- Vascular pharmacology [ 1537-1891 ]
Descripteurs français
- KwdFr :
- ADN (génétique), Animaux, Antigènes CD34 (génétique), Débit sanguin régional (physiologie), Facteur de croissance endothéliale vasculaire de type C (génétique), Imagerie par résonance magnétique, Immunohistochimie, Lymphangiogenèse (génétique), Lymphangiogenèse (physiologie), Lymphoedème (), Lymphoedème (génétique), Lymphoedème (imagerie diagnostique), Membre pelvien (), Membre pelvien (anatomopathologie), Mâle, Plasmides (génétique), Rat Wistar, Rats, Technique d'immunofluorescence, Techniques de transfert de gènes, Thérapie génétique, Échographie.
- MESH :
- anatomopathologie : Membre pelvien.
- génétique : ADN, Antigènes CD34, Facteur de croissance endothéliale vasculaire de type C, Lymphangiogenèse, Lymphoedème, Plasmides.
- imagerie diagnostique : Lymphoedème.
- physiologie : Débit sanguin régional, Lymphangiogenèse.
- Animaux, Imagerie par résonance magnétique, Immunohistochimie, Lymphoedème, Membre pelvien, Mâle, Rat Wistar, Rats, Technique d'immunofluorescence, Techniques de transfert de gènes, Thérapie génétique, Échographie.
English descriptors
- KwdEn :
- Animals, Antigens, CD34 (genetics), DNA (genetics), Fluorescent Antibody Technique, Gene Transfer Techniques, Genetic Therapy, Hindlimb (blood supply), Hindlimb (pathology), Immunohistochemistry, Lymphangiogenesis (genetics), Lymphangiogenesis (physiology), Lymphedema (diagnostic imaging), Lymphedema (genetics), Lymphedema (therapy), Magnetic Resonance Imaging, Male, Plasmids (genetics), Rats, Rats, Wistar, Regional Blood Flow (physiology), Ultrasonography, Vascular Endothelial Growth Factor C (genetics).
- MESH :
- chemical , genetics : Antigens, CD34, DNA, Vascular Endothelial Growth Factor C.
- blood supply : Hindlimb.
- diagnostic imaging : Lymphedema.
- genetics : Lymphangiogenesis, Lymphedema, Plasmids.
- pathology : Hindlimb.
- physiology : Lymphangiogenesis, Regional Blood Flow.
- therapy : Lymphedema.
- Animals, Fluorescent Antibody Technique, Gene Transfer Techniques, Genetic Therapy, Immunohistochemistry, Magnetic Resonance Imaging, Male, Rats, Rats, Wistar, Ultrasonography.
Abstract
Secondary lymphedema has been clinically well described, but a cure is still lacking. Although there have been previous investigations using plasmid DNA for gene therapy, few have focused on the use for the treatment of lymphedema. Therefore, we investigated the effects of VEGF-C gene transfer for the treatment of lymphedema using our plasmid pcDNA3.1-VEGF-C. We produced a surgical model of secondary lymphedema in the rat hindlimb and treated with local intradermal VEGF-C transfection to investigate the efficacy of gene transfer. Magnetic resonance imaging (MRI) (P<0.05), B ultrasound (P<0.05), and water displacement volumetry (P<0.05) demonstrated a reduction of lymphedema in therapy group as compared to controls. Histological and immunofluorescent studies demonstrated numerous newly formed lymphatic vessels in therapy group. Our results indicate that VEGF-C gene therapy has produced new lymphatic vessels which may have improved functional lymphatic drainage to reduce lymphedema volume in our model.
DOI: 10.1016/j.vph.2008.01.010
PubMed: 18455964
Affiliations:
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in rat model of secondary lymphedema.</title><author><name sortKey="Liu, Yanli" sort="Liu, Yanli" uniqKey="Liu Y" first="Yanli" last="Liu">Yanli Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Anatomy, Shandong University School of Medicine, Jinan, 250012, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Anatomy, Shandong University School of Medicine, Jinan, 250012</wicri:regionArea><wicri:noRegion>250012</wicri:noRegion></affiliation></author><author><name sortKey="Fang, Yunhai" sort="Fang, Yunhai" uniqKey="Fang Y" first="Yunhai" last="Fang">Yunhai Fang</name></author><author><name sortKey="Dong, Ping" sort="Dong, Ping" uniqKey="Dong P" first="Ping" last="Dong">Ping Dong</name></author><author><name sortKey="Gao, Jie" sort="Gao, Jie" uniqKey="Gao J" first="Jie" last="Gao">Jie Gao</name></author><author><name sortKey="Liu, Rong" sort="Liu, Rong" uniqKey="Liu R" first="Rong" last="Liu">Rong Liu</name></author><author><name sortKey="Hhahbaz M" sort="Hhahbaz M" uniqKey="Hhahbaz M" last="Hhahbaz M">Hhahbaz M</name></author><author><name sortKey="Bi, Yushun" sort="Bi, Yushun" uniqKey="Bi Y" first="Yushun" last="Bi">Yushun Bi</name></author><author><name sortKey="Ding, Zhaoxi" sort="Ding, Zhaoxi" uniqKey="Ding Z" first="Zhaoxi" last="Ding">Zhaoxi Ding</name></author><author><name sortKey="Tian, Hua" sort="Tian, Hua" uniqKey="Tian H" first="Hua" last="Tian">Hua Tian</name></author><author><name sortKey="Liu, Zhiyu" sort="Liu, Zhiyu" uniqKey="Liu Z" first="Zhiyu" last="Liu">Zhiyu Liu</name></author></analytic><series><title level="j">Vascular pharmacology</title><idno type="ISSN">1537-1891</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, CD34 (genetics)</term><term>DNA (genetics)</term><term>Fluorescent Antibody Technique</term><term>Gene Transfer Techniques</term><term>Genetic Therapy</term><term>Hindlimb (blood supply)</term><term>Hindlimb (pathology)</term><term>Immunohistochemistry</term><term>Lymphangiogenesis (genetics)</term><term>Lymphangiogenesis (physiology)</term><term>Lymphedema (diagnostic imaging)</term><term>Lymphedema (genetics)</term><term>Lymphedema (therapy)</term><term>Magnetic Resonance Imaging</term><term>Male</term><term>Plasmids (genetics)</term><term>Rats</term><term>Rats, Wistar</term><term>Regional Blood Flow (physiology)</term><term>Ultrasonography</term><term>Vascular Endothelial Growth Factor C (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (génétique)</term><term>Animaux</term><term>Antigènes CD34 (génétique)</term><term>Débit sanguin régional (physiologie)</term><term>Facteur de croissance endothéliale vasculaire de type C (génétique)</term><term>Imagerie par résonance magnétique</term><term>Immunohistochimie</term><term>Lymphangiogenèse (génétique)</term><term>Lymphangiogenèse (physiologie)</term><term>Lymphoedème ()</term><term>Lymphoedème (génétique)</term><term>Lymphoedème (imagerie diagnostique)</term><term>Membre pelvien ()</term><term>Membre pelvien (anatomopathologie)</term><term>Mâle</term><term>Plasmides (génétique)</term><term>Rat Wistar</term><term>Rats</term><term>Technique d'immunofluorescence</term><term>Techniques de transfert de gènes</term><term>Thérapie génétique</term><term>Échographie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, CD34</term><term>DNA</term><term>Vascular Endothelial Growth Factor C</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Membre pelvien</term></keywords><keywords scheme="MESH" qualifier="blood supply" xml:lang="en"><term>Hindlimb</term></keywords><keywords scheme="MESH" qualifier="diagnostic imaging" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lymphangiogenesis</term><term>Lymphedema</term><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term><term>Antigènes CD34</term><term>Facteur de croissance endothéliale vasculaire de type C</term><term>Lymphangiogenèse</term><term>Lymphoedème</term><term>Plasmides</term></keywords><keywords scheme="MESH" qualifier="imagerie diagnostique" xml:lang="fr"><term>Lymphoedème</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Hindlimb</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Débit sanguin régional</term><term>Lymphangiogenèse</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Lymphangiogenesis</term><term>Regional Blood Flow</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Fluorescent Antibody Technique</term><term>Gene Transfer Techniques</term><term>Genetic Therapy</term><term>Immunohistochemistry</term><term>Magnetic Resonance Imaging</term><term>Male</term><term>Rats</term><term>Rats, Wistar</term><term>Ultrasonography</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Imagerie par résonance magnétique</term><term>Immunohistochimie</term><term>Lymphoedème</term><term>Membre pelvien</term><term>Mâle</term><term>Rat Wistar</term><term>Rats</term><term>Technique d'immunofluorescence</term><term>Techniques de transfert de gènes</term><term>Thérapie génétique</term><term>Échographie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Secondary lymphedema has been clinically well described, but a cure is still lacking. Although there have been previous investigations using plasmid DNA for gene therapy, few have focused on the use for the treatment of lymphedema. Therefore, we investigated the effects of VEGF-C gene transfer for the treatment of lymphedema using our plasmid pcDNA3.1-VEGF-C. We produced a surgical model of secondary lymphedema in the rat hindlimb and treated with local intradermal VEGF-C transfection to investigate the efficacy of gene transfer. Magnetic resonance imaging (MRI) (P<0.05), B ultrasound (P<0.05), and water displacement volumetry (P<0.05) demonstrated a reduction of lymphedema in therapy group as compared to controls. Histological and immunofluorescent studies demonstrated numerous newly formed lymphatic vessels in therapy group. Our results indicate that VEGF-C gene therapy has produced new lymphatic vessels which may have improved functional lymphatic drainage to reduce lymphedema volume in our model.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Bi, Yushun" sort="Bi, Yushun" uniqKey="Bi Y" first="Yushun" last="Bi">Yushun Bi</name><name sortKey="Ding, Zhaoxi" sort="Ding, Zhaoxi" uniqKey="Ding Z" first="Zhaoxi" last="Ding">Zhaoxi Ding</name><name sortKey="Dong, Ping" sort="Dong, Ping" uniqKey="Dong P" first="Ping" last="Dong">Ping Dong</name><name sortKey="Fang, Yunhai" sort="Fang, Yunhai" uniqKey="Fang Y" first="Yunhai" last="Fang">Yunhai Fang</name><name sortKey="Gao, Jie" sort="Gao, Jie" uniqKey="Gao J" first="Jie" last="Gao">Jie Gao</name><name sortKey="Hhahbaz M" sort="Hhahbaz M" uniqKey="Hhahbaz M" last="Hhahbaz M">Hhahbaz M</name><name sortKey="Liu, Rong" sort="Liu, Rong" uniqKey="Liu R" first="Rong" last="Liu">Rong Liu</name><name sortKey="Liu, Zhiyu" sort="Liu, Zhiyu" uniqKey="Liu Z" first="Zhiyu" last="Liu">Zhiyu Liu</name><name sortKey="Tian, Hua" sort="Tian, Hua" uniqKey="Tian H" first="Hua" last="Tian">Hua Tian</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Yanli" sort="Liu, Yanli" uniqKey="Liu Y" first="Yanli" last="Liu">Yanli Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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